Tag: in-vivo

Accepted paper in Acta Biomaterialia on testing gold nanoparticles in vivo using C. elegans

The paper In vivo testing of gold nanoparticles using the Caenorhabditis elegans model organism” has been publised in Acta Biomaterialia (Available online 1 February 2017; doi: 10.1016/j.actbio.2017.01.080). 

The authors of the paper are Laura González-Moragas, Pascal Berto, Clara VilchesRomain Quidant, Androniki Kolovou, Rachel Santarella-Mellwig, Yannick Schwab, Stephen Stürzenbaum, Anna Roig, and Anna Laromaine

The paper is a result of a collaboration between the NN Group at ICMAB (González-Moragas, Roig and Laromaine), the ICFO-Institut de Ciències Fotòniques (Berto, Vilches and Quidant), the European Molecular Biology Laboratory, EMBL in Heidelberg, Germany (Kolovou, Santarella-Mellwig, Schwab) and King’s College London in UK (Stürzenbaum).  



Gold nanoparticles (AuNPs) are present in many man-made products and cosmetics, and are also used by the food and medical industries. Tight regulations regarding the use of mammalian animals for product testing can hamper the study of the specific interactions between engineered nanoparticles and biological systems. Invertebrate models, such as the nematode Caenorhabditis elegans (C. elegans), can offer alternative approaches during the early phases of nanoparticle discovery.

Panels B and C are optical microscopy images of B) 11-nm and C) 150-nm AuNPs treated worms. 11-nm AuNPs appear pink, and 150-nm AuNPs appear blue.

Here, we thoroughly evaluated the biodistribution of 11-nm and 150-nm citrate-capped AuNPs in the model organism C. elegans at multiple scales, moving from micrometric to nanometric resolution and from the organism to cellular level. We confirmed that the nanoparticles were not able to cross the intestinal and dermal barriers. We investigated the effect of AuNPs on the survival and reproductive performance of C. elegans, and correlated these effects with the uptake of AuNPs in terms of their number, surface area, and metal mass. In general, exposure to 11-nm AuNPs resulted in a higher toxicity than the larger 150-nm AuNPs. NP aggregation inside C. elegans was determined using absorbance microspectroscopy, which allowed the plasmonic properties of AuNPs to be correlated with their confinement inside the intestinal lumen, where anatomical traits, acidic pH and the presence of biomolecules play an essential role on NP aggregation. Finally, quantitative PCR of selected molecular markers indicated that exposure to AuNPs did not significantly affect endocytosis and intestinal barrier integrity.


Anna Roig at the HINT COST Action Final Meeting in Aveiro, Portugal

aveiroThe Final Meeting of the COST Action on Hybrid Interfaces (HINT) will take place on October 10-12, 2016, at the Reitoria building of the University of Aveiro, Portugal. The meeting aims at sharing the learned lessons on design, control, and dynamics of interfaces in hybrid organic-inorganic materials over the last four years in which the different partners have worked on this topics. 

Anna Roig will participate in the meeting and will give a talk on “Bio-screening of protein coated SPIONs: in vitro and in vivo by the C. elegans model” (Monday, October 10, 5.40 pm).

For more information on the agenda, please visit the conference webiste: http://costhint.web.ua.pt/